LncRNA CASC15 serves as a diagnostic biomarker for postmenopausal osteoporotic fractures and its knockdown enhances HBMSC osteogenic differentiation via miR-152-3p.

ABSTRACT:

Objective

Long non-coding RNA CASC15 plays a role in the development of various human diseases. However, the diagnostic role and molecular mechanisms of CASC15 in postmenopausal osteoporosis (PMOP) remain unclear.

Methods

This study enrolled 137 healthy postmenopausal women and 141 PMOP patients. CASC15 and miR-152-3p levels in serum and HBMSCs were measured via RT-qPCR. Western blotting and RT-qPCR were employed to analyze the protein and mRNA expression levels of osteogenic markers (RUNX2, ALP, OCN, Col I). CASC15's diagnostic value was assessed by the ROC curve. Proliferation (CCK-8) and apoptosis (flow cytometry) were examined. CASC15-miR-152-3p targeting was confirmed via DLR and RIP assays.

Results

CASC15 expression was increased, while miR-152-3p expression was

SUBMITTER: Wu X 

PROVIDER: S-EPMC12922429 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature