Detection of Multiple Microorganisms in Ruminant Ticks in Senegal Using High-Throughput Microfluidic Real-Time PCR.

ABSTRACT: Ticks are major vectors of numerous pathogens affecting both livestock and humans. In Senegal, data on the diversity of tick-borne pathogens (TBPs) in ruminant-associated ticks remain limited. In total, 1703 ticks were collected from goats, sheep, and cattle across three ecological zones of Senegal (Sudanian, Sahelian, and Sudano-Sahelian). Tick species were identified morphologically, and 300 individuals were screened for 36 microorganisms using a high-throughput microfluidic real-time PCR system. DNA was successfully extracted and amplified from 289 ticks. The most abundant species were Rhipicephalus evertsi evertsi (32.3%), Hyalomma truncatum (19.6%), R. guilhoni (15.6%), H. rufipes (11.6%), and Amblyomma variegatum (11.0%). Among the screened ticks, 2

SUBMITTER: Khoule A 

PROVIDER: S-EPMC12922542 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature