Statistical methods for predicting the presence of Salmonella Typhi in wastewater samples at Asante Akyem Agogo, Ghana.

ABSTRACT:

Background

Monitoring wastewater is vital for tracking typhoid fever in endemic areas. This study evaluated the performance of both spatial and non-spatial models in predicting Salmonella Typhi detection in wastewater from the Asante Akim North district in Ghana and identified key environmental risk factors.

Methods

We collected wastewater samples of Moore swabs at 40 sites across Agogo, Juansa, Hwidiem, and Domeabra over a period of 27 months. Multiplex PCR was used to detect Salmonella Typhi, focusing on the ttr, tviB, and staG genes. An Aquaprobe AP-2000 was also used to measure different physicochemical factors, such as pH, temperature, dissolved oxygen, and salinity. Three non-spatial models, namely Generalized Estimating Equations (Logistic), Mixed-Effects Models, and

SUBMITTER: Twumasi-Ankrah S 

PROVIDER: S-EPMC12923122 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature