Pharmaceutical Metabolite Data Base, PharmMet DB: Reference data base for drug metabolites generated by human liver S9 fraction.

ABSTRACT: Drug monitoring is an essential component of precision therapeutics, yet existing data bases to support therapeutic monitoring are limited to data curated from the scientific literature or predicted in silico. We used human liver S9 fraction to generate metabolites from 1114 therapeutic drugs spanning diverse drug classes. Metabolites were analyzed by liquid chromatography-high-resolution mass spectrometry, annotated through differential analysis of preincubation and postincubation samples, curated by comparison to predicted metabolites from BioTransformer 3.0, and compiled into a human liver pharmaceutical metabolite resource, named "Pharmaceutical Metabolite Data Base (PharmMet DB)." Liquid chromatography-high-resolution mass spectrometry showed heterogeneity in product generation, with

SUBMITTER: Jeon J 

PROVIDER: S-EPMC12923290 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature