Green synthesis of silver nanoparticles by isolated Bacillus velezensis BS1 and their applications.

ABSTRACT: This study aimed to isolate a local silver-resistant bacterial strain capable of efficiently synthesizing silver nanoparticles (AgNPs) and to optimize the parameters affecting bacterial growth and the bioreduction process. The biosynthesized AgNPs were characterized using UV-Vis spectroscopy, TEM, FTIR, and zeta potential analyses, and their biological activities were assessed through antibacterial, cytotoxicity, and seed germination assays. Bacillus velezensis BS1 was identified as the most promising isolate for AgNPs biosynthesis. Optimal bioreduction conditions were achieved at 70 °C and pH 9 after 3 h with 5 mM AgNO₃. The UV-Vis spectra exhibited surface plasmon resonance peaks at 410-450 nm, confirming the formation of AgNPs. The resulting AgNPs were spherical, negatively c

SUBMITTER: Aletrepy AA 

PROVIDER: S-EPMC12923455 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature