Intranasal dantrolene nanoparticles inhibit lipopolysaccharide-induced depression and anxiety behavior in mice.

ABSTRACT: This study investigates the therapeutic effectiveness of intranasal dantrolene nanoparticle pretreatment to inhibit lipopolysaccharide (LPS)-induced pathological inflammation, synapse destruction, and depressive and anxiety behavior in mice. B6SJLF1/J adult mice were pretreated with intranasal dantrolene nanoparticles (dantrolene: 5 mg/kg), daily, Monday to Friday, 5 days per week, for 4 weeks. Afterwards, mice were treated with a single intraperitoneal injection of LPS (5 mg/kg). Behavioral tests for depression and anxiety were performed 24 h after the one-time LPS injection. Biomarkers for pyroptosis-related inflammation cytokine levels (IL-1β and IL-18) in the blood and brain were measured using enzyme-linked immunosorbent assay (ELISA) and immunoblotting, respectively. Changes in prima

SUBMITTER: Liu J 

PROVIDER: S-EPMC12923542 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature