Oncogenic PI3Kα variants reveal graded conformational spectrum with mutation-specific cryptic pockets.

ABSTRACT: Cancer-prone alleles exhibit single hotspot mutations. However, the combination of a cancer hotspot and a weak or moderate mutation (the 'one-two punch' hypothesis) produces same-allele double variants with a significantly different and potentially graded clinical phenotypic spectrum. Oncogenic PI3Kα variants, which are also associated with benign tumors and neurodevelopmental disorders, offer statistical support for this model. Using atomistic molecular dynamics (MD) simulations, we revealed that PI3Kα variants with single and double mutations exhibit expanded conformational profiles. Double mutations significantly shift the conformational ensembles toward the active form-a more pronounced effect than a single mutation. These double mutants facilitate nSH2 release, iSH2 shift, and A-loop

SUBMITTER: Jang H 

PROVIDER: S-EPMC12923765 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature