METTL3-mediated m⁶A modification regulates CDKN1A to attenuate chronic sleep deprivation-induced cognitive impairment and neuronal apoptosis in rats.

ABSTRACT: Chronic sleep deprivation (CSD) can induce cognitive impairment, but its molecular mechanism remains unclear. In this study, initial m⁶A RNA sequencing of the hippocampal CA3 region in CSD rats, coupled with differential gene expression analysis of the total RNA fraction, revealed downregulation of METTL3, which was consistent with impaired performance in the Morris Water Maze (MWM) and confirmed by qRT-PCR and Western blot. Further investigation showed that, in HT-22 cells, METTL3 knockdown exacerbated rapamycin-induced apoptosis. RNA sequencing of METTL3-knockdown cells identified gene modules and specific differentially expressed genes associated with METTL3 loss. Differential expression analysis revealed that CDKN1A was significantly upregulated following METTL3 knockdown. Methylated R

SUBMITTER: Xing F 

PROVIDER: S-EPMC12923855 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature