Combining multiplex metabolic engineering with adaptive evolution strategies for high-level succinic acid production in <i>Yarrowia lipolytica</i>.

ABSTRACT: Succinic acid, an essential platform chemical with extensive utility in biodegradable materials, pharmaceuticals, and the food industry, faces challenges of high energy consumption and environmental pollution in traditional chemical synthesis. Here, we employed multiplex metabolic engineering and adaptive laboratory evolution to enhance succinic acid biosynthesis in Y arrowia lipolytica. By attenuating succinate dehydrogenase (Sdh) activity, mitigating by-product accumulation, and enhancing the succinate synthesis pathway, engineered strains showed efficient succinic acid production from glycerol. The titer reached 130.99 g/L under unregulated pH conditions, translating to a yield of 0.35 g/g and a productivity of 0.70 g/(L·h). Subsequently, transporter engineering and adapti

SUBMITTER: Sun T 

PROVIDER: S-EPMC12923921 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature