Spatial single-cell analysis reveals tumor microenvironment signatures predictive of oral cavity cancer outcome.

ABSTRACT: Up to 20% of patients with early-stage oral squamous cell carcinoma (OSCC) develop postoperative relapse, but no parameters currently enable the identification of individuals with poor prognosis. Here, we report a spatially resolved single-cell analysis of the tumor microenvironment in OSCC. By analyzing >700,000 cells for 25 proteins using imaging mass cytometry, we discover leukocyte and endothelial cell phenotypes that are independent prognostic factors for survival. Most notably, the presence of proliferating lymphatic endothelial cells (KI67⁺PROX1⁺), which show distinct molecular signature in transcriptomic analyses, in the invasive tumor margin strongly predicts poor recurrence-free survival in early-stage OSCC. We validate our findings in an independent OSCC co

SUBMITTER: Nasiaho J 

PROVIDER: S-EPMC12923947 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature