CAR-T cells with the CD38^-CD73^-Tim-3^-HLA-DR⁺ phenotype predict the efficacy of tisagenlecleucel as a treatment for B cell precursor ALL.

ABSTRACT: Anti-CD19 chimeric antigen receptor T cell (CAR-T) therapy is highly effective for B cell precursor acute lymphoblastic leukemia (BCP-ALL); however, approximately half of the patients relapse. Thus, there is an urgent need to identify factors that improve efficacy. This study enrolls 19 patients with BCP-ALL (16 children and 3 young adults) who receive tisagenlecleucel. Infusion products, peripheral blood, and bone marrow samples are obtained before and after CAR-T cell infusion. Single-cell analysis reveals that central memory CAR^pos T cells increase in long-term responders, whereas CXCR3⁺CD38^highPD-1^high effector CAR^pos T cells are enriched in relapsed patients, post-infusion. By contrast, CAR^pos T cells obtained from infu

SUBMITTER: Mikami T 

PROVIDER: S-EPMC12923955 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature