Esophageal Cancer Cells Exhibit Heterogeneity in DNA Double-Strand Break Repair and G2/M Checkpoint Arrest Associated With Cell Viability After Ionizing Radiation.

ABSTRACT:

Purpose

Esophageal cancer lacks characteristic mutations in DNA repair genes; therefore, DNA damage response (DDR) factors have not been widely explored as predictive biomarkers in esophageal cancer. In this study, we explored the potential heterogeneity of DDR capabilities following exposure to ionizing radiation (IR).

Methods and materials

DNA repair protein RAD51 homolog 1/Rad51 recombinase (RAD51), breast cancer susceptibility gene 1, and replication protein A foci formation were analyzed in 15 esophageal cancer cell lines after IR. DNA damage signaling, including phosphorylation of ataxia telangiectasia mutation, Chk2, and Chk1, was examined by immunoblotting. G2/M checkpoint arrest after IR was assessed by scoring mitotic cells. The mode of cell death and cell viabilit

SUBMITTER: Tateno K 

PROVIDER: S-EPMC12924126 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature