Highly sensitive protein detection by aptamer-based single-molecule kinetic fingerprinting.

ABSTRACT: Sensitive assays of protein biomarkers play critical roles in clinical diagnostics and biomedical research. Such assays typically employ immunoreagents such as monoclonal antibodies that suffer from several drawbacks, including relatively tedious production, significant batch-to-batch variability, and challenges in site-specific, stoichiometric modification with fluorophores or other labels. One proposed alternative to such immunoreagents, nucleic acid aptamers generated by systematic evolution of ligand by exponential enrichment (SELEX), can be chemically synthesized with much greater ease, precision, and reproducibility than antibodies. However, most aptamers exhibit relatively poor affinity, yielding low sensitivity in the assays employing them. Recently, single molecule recognition thr

SUBMITTER: Chatterjee T 

PROVIDER: S-EPMC12924676 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature