YBX1 promotes angiogenesis after myocardial infarction by stabilizing HIF1α mRNA via m⁶A signaling.

ABSTRACT: Angiogenesis is essential for myocardial repair after myocardial infarction (MI). While Y-box binding protein 1 (YBX1) is well-established in cancer biology, its role in post-MI angiogenesis remains unknown. Here, we show that YBX1 expression is increased in endothelial cells after MI, with higher levels observed in the peri-infarct region, and is also induced in hypoxic HUVECs. In vitro, YBX1 overexpression enhances HUVEC viability, migration, and proliferation, whereas siRNA-mediated knockdown impairs these functions. In vivo, endothelial-specific AAV9-YBX1 delivery improves cardiac function, reduces fibrosis, and enhances angiogenesis in murine MI models, while AAV9-shYBX1 exacerbates cardiac injury. Mechanistically, RNA sequencing reveals that YBX1 deficiency disrupts HIF

SUBMITTER: Bi F 

PROVIDER: S-EPMC12924730 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature