Mass Spectrometry-Based Quantification of Proteins and Post-Translational Modifications in Dried Blood: Longitudinal Sampling of Patients With Sepsis in Tanzania.

ABSTRACT: The proteomic analysis of blood is routine for disease phenotyping and biomarker development. Blood is commonly separated into soluble and cellular fractions. However, this can introduce pre-analytical variability, and analysis of a single component (which is common) may ignore important pathophysiology. We have recently developed methods for the facile processing of dried blood for mass spectrometry-based quantification of the proteome, N-glycoproteome, and phosphoproteome. Here, we applied this approach to 38 patients in Tanzania who presented to the hospital with sepsis. Blood was collected on Mitra devices at presentation and 1, 3, and 28-42 days post-enrollment. Processing of 96 total samples was performed in plate-based formats and completed within 2 days. Approximately 2000 protein

SUBMITTER: Foster MW 

PROVIDER: S-EPMC12925604 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature