Structural insights into lacto-N-biose I recognition by a family 32 carbohydrate-binding module from Bifidobacterium bifidum.

ABSTRACT: Bifidobacterium bifidum, a predominant colonizer of the infant gut, utilizes lacto-N-biose I (LNB), a prominent component of human milk oligosaccharides (HMOs), through a dedicated metabolic pathway. Among a diverse set of extracellular glycosidases involved in HMO degradation, lacto-N-biosidase (LnbB) plays a pivotal role by releasing LNB. We investigated the structure and function of the carbohydrate-binding module family 32 (CBM32) domain located at the C-terminus of the glycoside hydrolase family 20 catalytic domain in LnbB. Isothermal titration calorimetry showed that CBM32 binds LNB with a dissociation constant (K_d) of 98 μm. The crystal structure of the CBM32 complexed with LNB reveals the molecular basis for its specific recognition. Impact statement Bifidobacteria are b

SUBMITTER: Zhang X 

PROVIDER: S-EPMC12926856 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature