Attomolar Sensitivity in Single Biomarker Counting upon Aqueous Two-Phase Surface Enrichment.

ABSTRACT: From longstanding techniques like enzyme-linked immunosorbent assay (ELISA) to modern next-generation sequencing, many of the most sensitive and specific biomarker detection assays require capture of the analyte at a surface. While surface-based assays provide advantages, including the ability to reduce background by washing away excess reagents and/or increase specificity through analyte-specific capture probes, the limited efficiency of capture from dilute solution often restricts assay sensitivity to the femtomolar-to-nanomolar range. Although assays for many nucleic acid analytes can decrease limits of detection (LODs) to the subfemtomolar range using polymerase chain reaction, such amplification may introduce biases, errors, and an increased risk of sample cross-contamination. Further

SUBMITTER: Li Z 

PROVIDER: S-EPMC12926873 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature