Molecular Biointerface Characterization for an Implanted Medical Device Using Cryogenic Orbitrap Secondary Ion Mass Spectrometry (Cryo-OrbiSIMS).

ABSTRACT: Implanted medical devices often fail due to foreign body reactions (FBRs), a process that is still not fully understood. This work presents a depth profiling approach to provide insight into the spatial metabolomics of the biointerface of implants, revealing biomolecular strata representative of the host response. This study examines silicone rubber poly(dimethylsiloxane) catheters implanted in mice for 1 and 28 days. Cryo-OrbiSIMS was used in combination with ToF-SIMS to identify metabolite profiles from the biological deposit found on the implants after removal from the tissue, which were previously unattainable using tissue sectioning. Machine learning and statistical analysis of the profiles were used to help identify early biointerface responses to the implant, including the observati

SUBMITTER: Bin Sabri AH 

PROVIDER: S-EPMC12926936 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature