Localisation of corticosteroids in male mouse kidney by mass spectrometry imaging.

ABSTRACT:

Graphical abstract

Abstract

Renal sodium balance is important for blood pressure homoeostasis and is regulated by corticosteroids, chiefly aldosterone but also glucocorticoids. Abundance of these hormones in functional subregions of the kidney is unknown, previous work being limited to measurements in plasma and urine, and in microdialysate from kidney medulla. Here, mass spectrometry imaging (MSI) was applied to map corticosteroids in kidney to understand how their distribution overlays with functional targets. Male C57BL/6J mice, aged 10 weeks, were fed diets containing 0.03, 0.3 or 3% w:w sodium for two weeks, and kidneys harvested at cull. Steroids were localised as Girard T derivatives on cryosections using matrix-assisted laser desorption ionisation Fourier transform

SUBMITTER: Stasinopoulos I 

PROVIDER: S-EPMC12927002 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature