Differentially Expressed Salivary miRNAs in Temporomandibular Disorders.

ABSTRACT:

Objective

Temporomandibular disorders (TMDs) are heterogeneous conditions of unclear aetiology involving the temporomandibular joint, masticatory muscles and neural tissues. Limited understanding of their pathogenesis hampers accurate diagnosis and targeted treatment. Therefore, this study aimed to identify salivary microRNA (miRNA) signatures associated with TMDs to support future diagnostic, therapeutic and prognostic applications.

Materials and methods

Unstimulated cell-free saliva (5 mL) was collected from 9 adult female TMD subjects (using Diagnostic Criteria/TMD) and eight healthy female controls of similar ages. Total RNA was extracted, small RNA libraries were prepared, and sequencing was performed using Illumina NovaSeq 6000. Reads were aligned to the human genome (

SUBMITTER: Kaczor-Urbanowicz KE 

PROVIDER: S-EPMC12927133 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature