Hypoxia Promotes an Adrenergic to Mesenchymal Transcriptional Program Transition in Neuroblastoma.

ABSTRACT: Adrenergic (ADRN) neuroblastoma cells have a paradoxical increase in TET1 expression and 5-hydroxymethylcytosine (5-hmC) deposition in hypoxia. However, the effect of hypoxia on the mesenchymal (MES) lineage remains less well understood. To identify lineage specific hypoxia responses, we evaluated 5-hmC and RNA expression in a panel of ADRN and MES cells cultured in normoxic or hypoxic conditions. In hypoxia, MES cells had only a modestly elevated expression of hypoxia signaling and no phenotypic changes. In contrast, ADRN cells showed increased 5-hmC deposition and decreased mitochondrial function in hypoxia. In hypoxia, only ADRN cells had increased expression of MES signature genes (p < 0.05) and related pathways including TNF-α signaling. Publicly available RNA-Seq data of diagnostic t

SUBMITTER: Chaves GS 

PROVIDER: S-EPMC12928030 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature