Functional Characterization of Organoids Derived From Irreversibly Damaged Liver of Patients With NASH.

ABSTRACT:

Background and aims

NASH will soon become the leading cause of liver transplantation in the United States and is also associated with increased COVID-19 mortality. Currently, there are no Food and Drug Administration-approved drugs available that slow NASH progression or address NASH liver involvement in COVID-19. Because animal models cannot fully recapitulate human NASH, we hypothesized that stem cells isolated directly from end-stage liver from patients with NASH may address current knowledge gaps in human NASH pathology.

Approach and results

We devised methods that allow the derivation, proliferation, hepatic differentiation, and extensive characterization of bipotent ductal organoids from irreversibly damaged liver from patients with NASH. The transcriptomes of organoid

SUBMITTER: McCarron S 

PROVIDER: S-EPMC12928191 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature