Multiplex PCR to Differentiate Monkeypox Virus Clades.

ABSTRACT: We designed a multiplex quantitative PCR to differentiate monkeypox virus clades. For clinical samples collected in the United Kingdom and Nigeria, sensitivity was 78% (95% CI 67.67%-86.14%) and specificity 94% (95% CI 80.84%-99.30%); for samples with cycle thresholds <35, sensitivity was 98% (95% CI 91.72%-99.96%) and specificity 94% (95% CI 80.84%-99.30%).

SUBMITTER: Williams CT 

PROVIDER: S-EPMC12928233 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature