DNA and RNA-based amplicon sequencing of paired supragingival and dentin lesion plaque in children with severe early childhood caries.

ABSTRACT: Early childhood caries is a form of tooth decay in children under the age of 6 and impacts more than 514 million children worldwide. Severe early childhood caries (S-ECC) represents the most advanced and aggressive form of early childhood caries, defined by an age-dependent number of cavitated, missing, or filled smooth surfaces in the primary maxillary anterior teeth, or an overall count of decayed, missing, or filled tooth surfaces. To advance our understanding of the microbiological differences between different types of dental caries, we sought to investigate deep dentin caries isolated from 13 children with S-ECC and compare it with supragingival plaque isolated from the same site to identify differentially abundant species. Co-extraction methods allowed species identification by both

SUBMITTER: Naumann AA 

PROVIDER: S-EPMC12929799 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature