Rapid Generation of Fusable Cell Beads for Multi-Scale Human Living Materials Assembly.

ABSTRACT: Three-dimensional self-assembled cellular aggregates, such as spheroids, provide unique building blocks for bottom-up tissue engineering and in vitro disease modeling. Nevertheless, traditional spheroid production methods require prolonged cell aggregation times and are highly dependent on cell type, requiring frequent optimization steps. Additionally, spheroids' size is dependent on their cell density, preventing a control over their final volume. Herein, a methodology combining metabolic glycoengineering and click chemistry with superhydrophobic surfaces is described to rapidly create spherically structured living bead units, that can surpass the fabrication constraints of conventional spheroids. Compared to spheroids produced in low attachment settings, the living beads comprising vario

SUBMITTER: Moura BS 

PROVIDER: S-EPMC12929925 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature