STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation.

ABSTRACT: The present research explored the contributions of signal transducer and activator of transcription 1 (STAT1) and tubulin β4A (TUBB4A) in melanoma pathogenesis, focusing on their roles in modulating cellular proliferation, motility and apoptotic pathways. The goal of the study was to establish foundational evidence of the role of these proteins in melanoma for the development of precision therapeutic interventions. Gene silencing approaches were utilized to suppress STAT1 expression, while TUBB4A overexpression was achieved both in vitro and in a murine xenograft model. Cellular proliferation was evaluated via Cell Counting Kit‑8 and colony formation assay, whereas migration capacity was assessed using Transwell migration assays. Apoptotic activity was quantified by flow cytometry u

SUBMITTER: Zhao R 

PROVIDER: S-EPMC12930139 | biostudies-literature | 2026 Apr

REPOSITORIES: biostudies-literature