Spatial Profiling of Germinal Centers in Mouse Secondary Lymphoid Organs Using MACSima Imaging Cyclic Staining Technology.

ABSTRACT: We developed a 50-plex imaging protocol using the MACSima platform to characterize the microarchitecture of germinal centers in secondary lymphoid tissues. This workflow combines tissue processing, automated cyclic imaging, image preprocessing, and a new analysis method that detects morphological changes in irregularly shaped stromal cells.

SUBMITTER: Molina ISM 

PROVIDER: S-EPMC12930330 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature