Engineering Pseudomonas taiwanensis VLB120 for regio- and stereospecific hydroxylation of L-lysine fueled by the Weimberg pathway.

ABSTRACT:

Background

Hydroxy-L-lysines are versatile chiral building blocks and can be obtained by hydroxylation of the amino acid L-lysine. The conversion is catalyzed by α-ketoglutarate-dependent lysine dioxygenases (KDOs), which belong to the superfamily of Fe²⁺/α-ketoglutarate-dependent oxygenases. These enzymes are highly regio- and stereoselective; however, they require α-ketoglutarate (α-KG) as a cosubstrate. Apart from the costly direct addition of α-KG, it can be generated via cellular metabolism from inexpensive and renewable carbon sources, such as D-xylose. Therefore, we engineered a Pseudomonas taiwanensis VLB120 chassis to efficiently convert L-lysine to hydroxy-L-lysine using KDOs with the supply of α-KG from D-xylose as the sole carbon source via the Weimberg pathw

SUBMITTER: Nerke P 

PROVIDER: S-EPMC12930750 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature