Single-Target Pairing System (StarPair) for Large-Scale Interrogation of Cell-Cell Interactions.

ABSTRACT: Coordination of cell populations through intercellular interactions is essential to the formation and function of living organisms. However, technologies enabling systematic dissection of cell-cell communications or manipulation of cell-cell interactions at the single-cell resolution remain largely out of reach. To solve this, a single-target pairing system, termed StarPair, is proposed, for the high-throughput and high-precision combination of desired single targets in droplets. How target buoyancy, target concentration, sorting parameters, droplet flow rates, and electric stimulation collectively determine the single-target pairing performance is comprehensively explored. Upon optimization of these parameters, pairing efficiencies over 95% can be achieved with an operation frequency reac

SUBMITTER: Mao T 

PROVIDER: S-EPMC12931200 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature