Metagenomic insights into the urban-rural variation of antimicrobial resistance and pathogen reservoirs in untreated wastewater from central India.

ABSTRACT:

Introduction

Rapid and scalable surveillance of antimicrobial resistance (AMR) is urgently needed in resource-constrained countries where routine monitoring is limited. Wastewater-based metagenomics offers a potential solution for early detection and geographic mapping of AMR.

Methods

We conducted a retrospective DNA shotgun metagenomic analysis of untreated wastewater collected across Nagpur, India (February-April 2021). A total of 422 grab samples were pooled into 138 composite samples from 10 urban zones and rural catchments. The bacterial microbiota and resistome were profiled, and urban-rural patterns were compared using diversity metrics and correlation analyses.

Results

Across all samples, 871 bacterial genera were detected, dominated by Proteobacteria, with fr

SUBMITTER: Secker B 

PROVIDER: S-EPMC12932555 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature