The small non-coding RNA sRNA102 regulates <i>Pseudomonas aeruginosa</i> virulence and host immunity by targeting the T3SS component PcrG.

ABSTRACT: This study investigates the regulatory role of a functionally under-characterized small non-coding RNA (sRNA), sRNA102, in Pseudomonas aeruginosa, focusing on its mechanisms of influencing bacterial virulence and host immune modulation. Using an in vivo murine intraperitoneal infection model and transcriptomic sequencing, we found that the expression of sRNA102 is host immune-dependent: its expression was significantly upregulated by approximately 3-fold in immunocompetent mouse infection models, whereas no significant upregulation was observed in immunodeficient mouse models. This trend was further validated in in vitro PAO1-immune cell co-culture systems and a whole-blood infection model. Functional studies demonstrated that sRNA102 enhances hemolytic activity by 2.5

SUBMITTER: Chen Y 

PROVIDER: S-EPMC12932574 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature