Novel histological observation method using the scattered light of unstained collagen sections.

ABSTRACT: Collagen fibers are significant constituents of connective tissues such as the skin, bone, and tendons. Their morphology and alignment are essential for understanding tissue function and pathology. Collagen fibers are conventionally observed using optical and electron microscopy, and Second-harmonic generation microscopy. This study introduces a novel microscopic imaging technique, Scattering Angle-Resolved Bioimaging (SARB), which utilizes the scattering properties of unstained specimens. The SARB is based on a conventional optical microscope with a structured illumination module that projects a checker pattern onto the sample. This allows the acquisition of images separated by the scattering angle, enabling the visualization of the scattering characteristics. We applied SARB and electron

SUBMITTER: Otaki M 

PROVIDER: S-EPMC12932632 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature