Automated mapping of DNA replication fork progression in human cells with ForkML.

ABSTRACT: Current approaches to mapping fork progression in the human genome suffer from drastically low throughput. Here, we introduce ForkML, a nanopore sequencing-based method automatically positioning thousands of individual fork velocities by tracking BrdU incorporation into replicating DNA after double pulse-labelling of asynchronous cells. ForkML recovers known human fork speed, accurately detects replication stress, and, crucially, connects replication dynamics to genomic and chromatin contexts, exposing fork slowdown in early-replicating transcribed regions.

SUBMITTER: Rojat V 

PROVIDER: S-EPMC12932727 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature