A Cre-mediated copy number variant compromises the reliability of a <i>LoxP-STOP-LoxP</i>-<i>PLAG1</i> driven brain tumor model.

ABSTRACT:

Background

The developmental context in which genetic alterations occur is crucial to understand disease progression. In pediatric cancer, modeling tumor formation in the right cell type is necessary to faithfully recapitulate the unique nature of pediatric tumors. The Cre-LoxP system is a powerful tool to modulate gene expression in specific cell types at discrete developmental time windows.

Methods

We used Cre-LoxP mouse models to study the role of the oncofetal transcription factor PLAG1 in pediatric brain tumor formation. We characterized our model using histology, DNA methylation based copy number variant (CNV) analysis on fresh frozen and FFPE derived samples, RNA sequencing, whole genome sequencing and whole genome CRISPR Cas9 screening.

Results

We gener

SUBMITTER: Vaillant J 

PROVIDER: S-EPMC12932942 | biostudies-literature | 2026 Jan-Dec

REPOSITORIES: biostudies-literature