Dexter Energy Transfer Photocatalytic Microdissection of Amyloid Plaques in Alzheimer's Disease.

ABSTRACT: Amyloid plaque is a pathological hallmark in Alzheimer's disease (AD) brain with its composition associated with disease etiology. Here, we report a photocatalyst, namely, AmyCAT, that selectively binds to amyloid fibrils in AD brain tissues and undergoes Dexter energy transfer (DET) to catalyze carbene production for amyloid proximity labeling. First, we designed the AmyCAT photocatalyst to universally bind different types of amyloids. Second, we energetically match the DET effect in a 12 × 4 photocatalyst-substrate array to activate the diazo substrate into reactive carbene species by visible green light (530-545 nm). Further, we demonstrate that the optimal AmyCAT probe catalyzes pan-amyloid protein labeling with a 30-fold selectivity over folded counterparts. Mechanistic studies confir

SUBMITTER: Sun R 

PROVIDER: S-EPMC12933367 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature