Human TMIGD1 Promoter Exhibits Robust Activity in Prokaryotic Cells.

ABSTRACT: Gene transcription in eukaryotes is orchestrated by intricate regulatory architectures that depend on chromatin context and a diverse repertoire of transcription factors. Consequently, eukaryotic promoters are thought to be incompatible with the streamlined transcriptional machinery of bacteria. Here, we challenge this assumption by demonstrating that a minimal 90-bp fragment of the human TMIGD1 promoter functions as an active, constitutive promoter in Escherichia coli. This compact human sequence drives reproducible reporter expression at levels comparable to established moderate-strength bacterial promoters, revealing an unexpected convergence between human and bacterial transcriptional recognition mechanisms. The ability of a human promoter to operate across domains of life sugge

SUBMITTER: Meyer R 

PROVIDER: S-EPMC12933629 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature