M5C: Novel Diagnostic and Drug Repurposing Targets for Nonalcoholic Steatohepatitis.

ABSTRACT:

Background

Epigenetic medication, such as RNA 5-methylcytosine (m5C), is well-recognized as a key regulator in hepatic metabolism and immune responses. However, m5C regulatory mechanisms in NASH pathogenesis have not yet been clearly elucidated.

Methods

By utilizing three bulk profiles of NASH patients acquired from GEO and integrative bioinformatic pipelines, such as Limma framework, consensus clustering, and machine learning, we first identified m5C-related molecular subgroups and hub genes for NASH patients. Besides, diagnostic performance and biological characteristics of m5C-related hub gene were estimated at bulk level. Indeed, the heterogeneity of m5C-related hub gene for NASH patients was deciphered in single-cell transcriptomic profiles at temporal and spatial manne

SUBMITTER: Chen S 

PROVIDER: S-EPMC12933632 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature