Annexin A2-dependent extracellular vesicle proteome modulates pre-metastatic stromal fibroblast behavior in triple-negative breast cancer.

ABSTRACT:

Background

Triple-negative breast cancer (TNBC) is an aggressive subclass of breast cancer with limited treatment options and a strong tendency to metastasize to the lung. Tumor-derived extracellular vesicles (EVs) are key mediators of stromal reprogramming in the pre-metastatic niche. Annexin A2 (AnxA2) is overexpressed in TNBC and linked to signal transduction, cytoskeletal remodeling, and poor prognosis, but its role in shaping EV cargo composition and consequent stromal behavior remains unclear.

Methods

EVs were isolated from the conditioned media of MDA-MB-4175 (LM2) TNBC cells with stable AnxA2 knockdown and control cells using differential ultracentrifugation. EVs were characterized and validated using nanoparticle tracking analysis, Western blotting, and cryo-electro

SUBMITTER: Trivedi R 

PROVIDER: S-EPMC12934012 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature