Impact of mineral oil overlay on immature oocyte maturation in an optimized in vitro culture system.

ABSTRACT:

Background

To assess the impact of oil overlay during maturation of immature oocytes in vitro in our optimized liquid culture system.

Methods

We conducted comparative analyses of pH, osmolality, and the levels of melatonin, FSH, E2, and hCG in the culture medium between oil-covered group (OC group) and non-oil-covered group (non-OC group). Furthermore, germinal vesicle (GV) oocytes were collected and matured into MII phase in our culture medium for 24 h. Subsequently, levels of ROS, SOD, MDA, and LDH were tested in OC group and non-OC group using either microplate assays or chemical fluorescence techniques. Finally, a total of 2432 immature oocytes obtained from controlled ovarian hyperstimulation (COH) cycles underwent in vitro maturation (IVM) in culture media with or with

SUBMITTER: Peng J 

PROVIDER: S-EPMC12934039 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature