Methyltransferase-like 14 suppresses the retinal neovascularization by reducing HIF-1α in proliferative retinopathy.

ABSTRACT:

Background

Proliferative retinopathy (PR), a leading cause of visual impairment, is characterized by pathological retinal neovascularization. As an important methyltransferase, methyltransferase-like 14 (METTL14) plays a key role in the N6-methyladenosine (m⁶A) modification, which is the most widespread modification in mRNA and has been defined as a critical regulator in retinal diseases.

Methods

This study aims to clarify the mechanisms by which METTL14 regulates pathological retinal neovascularization in PR. The m⁶A levels were determined by m⁶A RNA colorimetric quantification in mice retinas and endothelial cells. The METTL14 levels in mice retinas and endothelial cells were detected by qPCR, western blotting and immunofluorescence assays.

SUBMITTER: Guan J 

PROVIDER: S-EPMC12934052 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature