METTL3 Potentiates Corneal Allograft Rejection via Increased Pro-Inflammatory Cytokine Production and Aerobic Glycolysis.

ABSTRACT:

Purpose

Allograft rejection is a primary cause of corneal transplantation failure. This study aims to investigate the role and underlying mechanisms of methyltransferase-like 3 (METTL3) in corneal allograft rejection.

Methods

The expression of N6-methyladenosine (m6A)-related genes, including Mettl3, in corneal grafts was examined using RNA sequencing, real-time PCR, and immunofluorescence staining. Genetic and pharmacological approaches were used to inhibit METTL3 activity, and corneal transplantation outcomes were evaluated via slit-lamp biomicroscopy, ELISA, real-time PCR, lactate assays, Seahorse metabolic assays, and Western blot. The effect of METTL3 on the inflammatory response was further examined in bone marrow-derived dendritic cells (BMDCs) using genetic and pharm

SUBMITTER: Yu H 

PROVIDER: S-EPMC12934518 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature