AMPK Deficiency Induces Corneal Epithelial Barrier Dysfunction by Modulating Energy Homeostasis.

ABSTRACT:

Purpose

This study aims to examine the impact of adenosine monophosphate-activated protein kinase (AMPK) on corneal epithelial barrier function and energy metabolism.

Methods

Corneal epithelial specific AMPK knockout mice were constructed to evaluate ocular surface phenotypes. In vitro experiments were performed using human corneal epithelial cells (HCECs) transfected with small interference RNA. Epithelial barrier function was assessed by analyzing apical junction complexes through immunofluorescence and Western blot in both in vivo and in vitro models. Changes in energy metabolism were evaluated by measuring oxygen consumption rate, mitochondrial dynamics protein expression, glucose uptake, and enzyme activity.

Results

Increased corneal fluorescein sodium staining a

SUBMITTER: Yang Y 

PROVIDER: S-EPMC12934520 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature