Histone Acetylation Landscape and the Role of HAT1 in Regulating Corneal Epithelial Wound Healing.

ABSTRACT:

Purpose

Histone acetyltransferase 1 (HAT1) plays critical roles in chromatin remodeling and gene expression; however, its specific role during corneal epithelial wound healing remains unknown. This study aimed to elucidate the function and underlying mechanisms of HAT1-mediated histone acetylation in corneal epithelial wound healing.

Methods

Murine corneal epithelial wound healing models were established using an Alger brush. Corneal epithelial-specific Hat1 conditional knockout mice were generated using the Cre-loxP system. Histone acetylation profile, including H4K5 acetylation (H4K5Ac), was assessed by Western blotting. HAT1 expression in human corneal epithelial cells was experimentally altered via small interfering RNA-mediated knockdown or vector-based overexpression.

SUBMITTER: Lin Y 

PROVIDER: S-EPMC12934522 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature