Biomass-Derived Diformylxylose as a Renewable Solvent for Biocatalysis Applications.

ABSTRACT: Developing sustainable biocatalytic processes requires alternative solvents that support enzyme activity while reducing environmental impact. This study explores the potential to use diformylxylose (DFX), a xylose-derived green solvent, as a cosolvent in enzymatic reactions, and compares its application to reaction outcomes in conventional solvents such as dimethyl sulfoxide (DMSO) and dimethylformamide (DMF). A comprehensive enzyme panel, including ketoreductases (KREDs), lipases as well as transaminases (TAs) and imine reductases (IREDs) was tested for activity and stability in DFX. In the green solvent, the selected KREDs and the immobilized lipase CalB retained high or even superior catalytic activity compared to conventional media, while the selected biocatalysts from other enzyme cla

SUBMITTER: Ozgen FF 

PROVIDER: S-EPMC12934546 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature