Genomic expression programs and the integration of the CD28 costimulatory signal in T cell activation.

ABSTRACT: Optimal activation of T cells requires effective occupancy of both the antigen-specific T cell receptor and a second coreceptor such as CD28. We used cDNA microarrays to characterize the genomic expression program in human peripheral T cells responding to stimulation of these receptors. We found that CD28 agonists alone elicited few, but reproducible, changes in gene expression, whereas CD3 agonists elicited a multifaceted temporally choreographed gene expression program. The principal effect of simultaneous engagement of CD28 was to increase the amplitude of the CD3 transcriptional response. The induced genes whose expression was most enhanced by costimulation were significantly enriched for known targets of nuclear factor of activated T cells (NFAT) transcription factors. This enhancemen

SUBMITTER: Diehn M 

PROVIDER: S-EPMC129348 | biostudies-literature | 2002 Sep

REPOSITORIES: biostudies-literature