Preclinical development of a well-defined semisynthetic glycoconjugate vaccine against <i>Haemophilus influenzae</i> type b.

ABSTRACT: Vaccines are the most effective method for preventing bacterial infections. Identification of the best hapten is an essential step in the development of third-generation Haemophilus influenzae type b (Hib) conjugate vaccines. A comprehensive understanding of the relationship between Hib oligosaccharide chain length and immunological activities remains elusive. A series of Hib capsular polysaccharide (CPS) oligosaccharide derivatives of different lengths were synthesized to establish carbohydrate structure-function relationships. Glycan microarray screening and immunological evaluation of oligosaccharides revealed that tetramer 4, comprising four repeated disaccharide units, is the key immunogenic epitope of CPS. The investigation into the influence of carrier proteins, adjuva

SUBMITTER: Han W 

PROVIDER: S-EPMC12935054 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature