Attenuation mechanisms of the P7-P8 live-attenuated <i>cyvirus cyprinidallo2</i> vaccine potentially involve apoptosis non-inhibition feature: insights into virus pathogenesis.

ABSTRACT: Cyvirus cyprinidallo2 (CyHV-2) is an alloherpesvirus and the causative agent of herpesviral haematopoietic necrosis in goldfish. Whole-genome sequence comparison of the developed live-attenuated vaccine P7-P8 with virulent CyHV-2 strains revealed seven single-nucleotide polymorphisms, five deletions and one inversion in the ORFs, which may be involved in attenuation. A start codon loss in ORF 113, a putative apoptosis-inhibition gene, was observed in the mutations. In vitro assays indicated that apoptosis-related genes were upregulated in cells inoculated with the vaccine or virulent virus compared to uninfected cells. However, the vaccine group showed increased phosphatidylserine externalization and DNA damage, suggesting the apoptosis-inducing properties of P7-P8. In the

SUBMITTER: Saito H 

PROVIDER: S-EPMC12935156 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature