Prolonged Release of IL-10 From Enzyme-Mediated Poly-l-(Tyrosine-co-Phenylalanine) Nanocrystals Enhances Stability and Modulates Inflammatory Responses.

ABSTRACT: Protease-catalyzed synthesis of a peptide composed of l-tyrosine and l-phenylalanine (2.2:1 M ratio) was achieved in a natural deep eutectic solvent (NADES) medium and subsequently employed to encapsulate the anti-inflammatory cytokine interleukin-10 (IL-10). IL-10 was loaded into enzyme-mediated peptide nanocrystals at doses of 200 and 400 ng, achieving stable nanocomposite formulations. Release studies were conducted in phosphate-buffered saline (PBS, pH 7.4) at 37°C under gentle agitation, revealing a sustained release profile extending up to 30-31 days, with an initial release of approximately 16% within the first 5 days and near-complete release between Days 10 and 25, depending on loading. Encapsulation effectively protected IL-10 from rapid degradation observed for the free cytokine

SUBMITTER: Landa-Tencle F 

PROVIDER: S-EPMC12936265 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature