Highly specific and super-sensitive Dot-ELISA and colloidal gold immunochromatographic strips for the detection of Burkholderia glumae and Burkholderia plantarii of Rice bacterial panicle blight.

ABSTRACT: Burkholderia glumae (B. glumae) and Burkholderia plantarii (B. plantarii) are primary causal agents of rice bacterial panicle blight (RBPB) and cause substantial yield losses in rice worldwide. Given their seed-borne transmission characteristics, quarantine status and destructive hazards, rapid, super-sensitive, highly specific on-site detection technologies are urgently needed. Here, using B. glumae Os48 and B. plantarii ZJ171 as immunogens, we prepared two highly specific and ultra-sensitive monoclonal antibodies (mAbs) against B. glumae (4A7 and 8C5) and two highly specific and ultra-sensitive mAbs against B. plantarii (12B5 and 14B3). We then developed dot-enzyme-linked immunosorbent assays (Dot-ELISA) and colloidal gold immunochromatographic strip (CGICS) assays for detecting B. gluma

SUBMITTER: Dong J 

PROVIDER: S-EPMC12936288 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature