High-dimensional single-cell analyses reveal neutrophil heterogeneity in guttate psoriasis.

ABSTRACT:

Background

Guttate psoriasis (GP) is associated with streptococcal throat infection. Neutrophils are the first immune cells to respond to Group A Streptococcal (GAS) infection, but detailed analysis of their contribution to GP pathogenesis is lacking. The study primarily focuses on phenotyping the neutrophils located at the site of inflammation in GP skin and understanding their specific contribution to the pathogenesis of the disease.

Methods

Here, we performed a comprehensive immunophenotypic and transcriptomic analysis of neutrophils from blood and inflamed GP skin using high-dimensional single-cell protein and RNA analyses. Ex vivo stimulation of neutrophils and co-culture with CD4⁺ T cells was performed to validate the function of neutrophil subsets upon GAS

SUBMITTER: Padhi A 

PROVIDER: S-EPMC12936745 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature